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primary antibody incubations  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology primary antibody incubations
    Primary Antibody Incubations, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+raptor/Raptor+Antibody/pm41826809-87-0-10
    Average 94 stars, based on 108 article reviews
    primary antibody incubations - by Bioz Stars, 2026-11
    94/100 stars

    Images

    Related Articles

    Generated:

    Article Title: Mammalian Target of Rapamycin Complex I (mTORC1) Activity in Ras Homologue Enriched in Brain (Rheb)-Deficient Mouse Embryonic Fibroblasts
    Article Snippet: .. anti-phospho-T389-S6K, anti-phospho-S235/236-S6, anti-RAPTOR, anti-mTOR, anti-PRAS40, anti-phospho-S473-PKB, anti-S6K, anti-4EBP1, anti-phospho-T37/46-4EBP1, anti- non-phospho-T46-4EBP1, anti-phospho-ERK1/2, anti-pAMPK T172, anti-eIF4E and anti-IRS-1 were from Cell Signaling; anti-phospho-PRAS40 S183 from IBL-America; anti-phospho-T246-PRAS40 from Invitrogen BioSource; anti-Raptor was from the University of Dundee (J. Hastie), anti-PKB was home-made; anti-GAPDH and anti-phospho-Raptor S863 were from Santa Cruz; anti-Rheb was a monoclonal antibody generated by R.F. ..

    SDS Page:

    Article Title: Diacylglycerol kinase η regulates C2C12 myoblast proliferation through the mTOR signaling pathway.
    Article Snippet: Diacylglycerol kinase (DGK) phosphorylates diacylglycerol to produce phosphatidic acid (PA).. The η isozyme of DGK is abundantly expressed in C2C12 myoblasts.. However, the role of DGKη in skeletal muscle cells remains unknown.

    Western Blot:

    Article Title: Diacylglycerol kinase η regulates C2C12 myoblast proliferation through the mTOR signaling pathway.
    Article Snippet: Diacylglycerol kinase (DGK) phosphorylates diacylglycerol to produce phosphatidic acid (PA).. The η isozyme of DGK is abundantly expressed in C2C12 myoblasts.. However, the role of DGKη in skeletal muscle cells remains unknown.

    other:

    Article Title: Amino Acid-Mediated Intracellular Ca 2+ Rise Modulates mTORC1 by Regulating the TSC2-Rheb Axis through Ca 2+ /Calmodulin
    Article Snippet: Anti-S6K1 (C-18, sc-230), anti-Raptor (10E10, sc-81537), anti-Rictor (H-11, sc-271081), anti-TSC2 (C-20, sc-893), anti-Myc (9E10, sc-40), and anti-GST (Z-5, sc-459) were from Santa Cruz Biotechnology (Dallas, TX, USA).

    Immunofluorescence:

    Article Title: mTORC1 signaling pathway integrates estrogen and growth factor to coordinate vaginal epithelial cells proliferation and differentiation.
    Article Snippet: Sections were permeabilized in 0.2% Triton-100 in PBS for 45min and then blocked with 1% (wt/vol) BSA Fraction V (ST023, Beyotime) and 10% Goat serum Cell Death and Disease (2022) 13:862 (vol/vol) (B900780, Proteintech) in PBS before the primary antibodies were added in immunohistochemical and immunofluorescence staining. .. For immunofluorescence staining, sections were incubated with the following primary antibodies: anti-Raptor (sc-81537, Santa Cruz Biotechnology), anti-Phospho-S6 Ribosomal Protein (Ser235/236) (62016, Cell Signaling Technology), anti-PR (9856 S, Cell Signaling Technology), anti-ERalpha Ab (ab32063, Abcam), anti-Ki67 Ab (ab15580, Abcam), anti-YAP1 Ab (13584-1-AP, Proteintech), anti-EREG Ab (MAB1068, R&D Systems) overnight at 4 °C. .. Followed by the incubation with the secondary antibodies: Alexa Fluor 488-conjugated affiniPure Goat anti-Rabbit IgG (H+ L) (115- 585-146, Jackson ImmunoResearch), Alexa Fluor 594-conjugated affiniPure Goat anti-Mouse IgG (H+ L) (111-545-144, Jackson ImmunoResearch) for 1 h, and nuclei-staining with DAPI (D9542, Sigma) for 10min at room temperature.

    Article Title: mTORC1 signaling pathway integrates estrogen and growth factor to coordinate vaginal epithelial cells proliferation and differentiation
    Article Snippet: Sections were permeabilized in 0.2% Triton-100 in PBS for 45 min and then blocked with 1% (wt/vol) BSA Fraction V (ST023, Beyotime) and 10% Goat serum (vol/vol) (B900780, Proteintech) in PBS before the primary antibodies were added in immunohistochemical and immunofluorescence staining. .. For immunofluorescence staining, sections were incubated with the following primary antibodies: anti-Raptor (sc-81537, Santa Cruz Biotechnology), anti-Phospho-S6 Ribosomal Protein (Ser235/236) (62016, Cell Signaling Technology), anti-PR (9856 S, Cell Signaling Technology), anti-ER-alpha Ab (ab32063, Abcam), anti-Ki67 Ab (ab15580, Abcam), anti-YAP1 Ab (13584-1-AP, Proteintech), anti-EREG Ab (MAB1068, R&D Systems) overnight at 4 °C. .. Followed by the incubation with the secondary antibodies: Alexa Fluor 488-conjugated affiniPure Goat anti-Rabbit IgG (H + L) (115-585-146, Jackson ImmunoResearch), Alexa Fluor 594-conjugated affiniPure Goat anti-Mouse IgG (H + L) (111-545-144, Jackson ImmunoResearch) for 1 h, and nuclei-staining with DAPI (D9542, Sigma) for 10 min at room temperature.

    Staining:

    Article Title: mTORC1 signaling pathway integrates estrogen and growth factor to coordinate vaginal epithelial cells proliferation and differentiation.
    Article Snippet: Sections were permeabilized in 0.2% Triton-100 in PBS for 45min and then blocked with 1% (wt/vol) BSA Fraction V (ST023, Beyotime) and 10% Goat serum Cell Death and Disease (2022) 13:862 (vol/vol) (B900780, Proteintech) in PBS before the primary antibodies were added in immunohistochemical and immunofluorescence staining. .. For immunofluorescence staining, sections were incubated with the following primary antibodies: anti-Raptor (sc-81537, Santa Cruz Biotechnology), anti-Phospho-S6 Ribosomal Protein (Ser235/236) (62016, Cell Signaling Technology), anti-PR (9856 S, Cell Signaling Technology), anti-ERalpha Ab (ab32063, Abcam), anti-Ki67 Ab (ab15580, Abcam), anti-YAP1 Ab (13584-1-AP, Proteintech), anti-EREG Ab (MAB1068, R&D Systems) overnight at 4 °C. .. Followed by the incubation with the secondary antibodies: Alexa Fluor 488-conjugated affiniPure Goat anti-Rabbit IgG (H+ L) (115- 585-146, Jackson ImmunoResearch), Alexa Fluor 594-conjugated affiniPure Goat anti-Mouse IgG (H+ L) (111-545-144, Jackson ImmunoResearch) for 1 h, and nuclei-staining with DAPI (D9542, Sigma) for 10min at room temperature.

    Article Title: mTORC1 signaling pathway integrates estrogen and growth factor to coordinate vaginal epithelial cells proliferation and differentiation
    Article Snippet: Sections were permeabilized in 0.2% Triton-100 in PBS for 45 min and then blocked with 1% (wt/vol) BSA Fraction V (ST023, Beyotime) and 10% Goat serum (vol/vol) (B900780, Proteintech) in PBS before the primary antibodies were added in immunohistochemical and immunofluorescence staining. .. For immunofluorescence staining, sections were incubated with the following primary antibodies: anti-Raptor (sc-81537, Santa Cruz Biotechnology), anti-Phospho-S6 Ribosomal Protein (Ser235/236) (62016, Cell Signaling Technology), anti-PR (9856 S, Cell Signaling Technology), anti-ER-alpha Ab (ab32063, Abcam), anti-Ki67 Ab (ab15580, Abcam), anti-YAP1 Ab (13584-1-AP, Proteintech), anti-EREG Ab (MAB1068, R&D Systems) overnight at 4 °C. .. Followed by the incubation with the secondary antibodies: Alexa Fluor 488-conjugated affiniPure Goat anti-Rabbit IgG (H + L) (115-585-146, Jackson ImmunoResearch), Alexa Fluor 594-conjugated affiniPure Goat anti-Mouse IgG (H + L) (111-545-144, Jackson ImmunoResearch) for 1 h, and nuclei-staining with DAPI (D9542, Sigma) for 10 min at room temperature.

    Incubation:

    Article Title: mTORC1 signaling pathway integrates estrogen and growth factor to coordinate vaginal epithelial cells proliferation and differentiation.
    Article Snippet: Sections were permeabilized in 0.2% Triton-100 in PBS for 45min and then blocked with 1% (wt/vol) BSA Fraction V (ST023, Beyotime) and 10% Goat serum Cell Death and Disease (2022) 13:862 (vol/vol) (B900780, Proteintech) in PBS before the primary antibodies were added in immunohistochemical and immunofluorescence staining. .. For immunofluorescence staining, sections were incubated with the following primary antibodies: anti-Raptor (sc-81537, Santa Cruz Biotechnology), anti-Phospho-S6 Ribosomal Protein (Ser235/236) (62016, Cell Signaling Technology), anti-PR (9856 S, Cell Signaling Technology), anti-ERalpha Ab (ab32063, Abcam), anti-Ki67 Ab (ab15580, Abcam), anti-YAP1 Ab (13584-1-AP, Proteintech), anti-EREG Ab (MAB1068, R&D Systems) overnight at 4 °C. .. Followed by the incubation with the secondary antibodies: Alexa Fluor 488-conjugated affiniPure Goat anti-Rabbit IgG (H+ L) (115- 585-146, Jackson ImmunoResearch), Alexa Fluor 594-conjugated affiniPure Goat anti-Mouse IgG (H+ L) (111-545-144, Jackson ImmunoResearch) for 1 h, and nuclei-staining with DAPI (D9542, Sigma) for 10min at room temperature.

    Article Title: mTORC1 signaling pathway integrates estrogen and growth factor to coordinate vaginal epithelial cells proliferation and differentiation
    Article Snippet: Sections were permeabilized in 0.2% Triton-100 in PBS for 45 min and then blocked with 1% (wt/vol) BSA Fraction V (ST023, Beyotime) and 10% Goat serum (vol/vol) (B900780, Proteintech) in PBS before the primary antibodies were added in immunohistochemical and immunofluorescence staining. .. For immunofluorescence staining, sections were incubated with the following primary antibodies: anti-Raptor (sc-81537, Santa Cruz Biotechnology), anti-Phospho-S6 Ribosomal Protein (Ser235/236) (62016, Cell Signaling Technology), anti-PR (9856 S, Cell Signaling Technology), anti-ER-alpha Ab (ab32063, Abcam), anti-Ki67 Ab (ab15580, Abcam), anti-YAP1 Ab (13584-1-AP, Proteintech), anti-EREG Ab (MAB1068, R&D Systems) overnight at 4 °C. .. Followed by the incubation with the secondary antibodies: Alexa Fluor 488-conjugated affiniPure Goat anti-Rabbit IgG (H + L) (115-585-146, Jackson ImmunoResearch), Alexa Fluor 594-conjugated affiniPure Goat anti-Mouse IgG (H + L) (111-545-144, Jackson ImmunoResearch) for 1 h, and nuclei-staining with DAPI (D9542, Sigma) for 10 min at room temperature.



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